IN VITRO THROMBOLYTIC / FIBRINOLYTIC EFFECTS OF RUE AQUEOUS DISTILLED EXTRACT

Abstract

Ruta graveolens showed several biological and pharmacological actions. In vivo, Its effect on the circulating blood is secondary rather than primary. The aim if this work was to study its effect on human blood clot, in vitro experimental model. This work was done in Department of Chemistry, College of Science, Baghdad University in Baghdad, Iraq. Aqueous distilled extract of Rue was prepared by simple distillation and scanned by UV-Visible spectrophotometer. The ability of this extract to generate peroxynitrite was investigated in vitro experimental mode. The effect of 100 μL of 1% Rue extract was tested on human blood clot prepared from five healthy volunteers. The extract was added either before the clot is formed or after it. UV-visible spectra of aqueous Rue extract showed the presence of single peak at 195nm.Rue extract enable to generate peroxynitrite in a concentration dependent manner (r = 0.98). It is significantly reduced the formed clot size by 12.534 ± 5.674% while the simultaneous administration of blood and aqueous extract resulted in reduction of clot size by 4.772 ± 2.207%. Rue aqueous distilled extract has thrombolytic and fibrinolytic effect by the evidence of clot lysis. This effect may be related to donation of nitric oxide.