تحديد الظروف المثلى لمؤشرات الـ (DAF (DAF Amplification Fingerprinting

Abstract

This type of markers was conducted for the first time, Where, much time was dispensed in performing many experiments reacted to optimal conditions for the amplification parameters, These also included the template DNA, primer, Magnesium ions, and enzyme concentrations. We found that the optimum concentration for: template DNA was 10 ng / reaction, the primer was 30 Pmol, Magnesium 6 mM, enzyme was 2.5 unit/ reaction.As well as the sensitive method use for detection of the amplified fragment by the polyacrylamide Gel electrophoresis which itself needed time for optimizing running and staining with AgNO3 conditions.