تأثير المستخلصات المائية والكحولية لنبات سم الفراخ على بعض عوامل الضراوة لبكتيريا Pseudomonas aeruoginosa المعزولة محلياً

Abstract

Two local isolates of Pseudomonas aeruginosa were selected the first isolate (PD1) was resistant to (11) antibiotics, and produced hemolysin, elastase and urease, the second isolate (PD2) was resistant to (4) antibiotics only (out of 12) and produced hemolysin, elastase but not urease.Minimum inhibitory concentration (MICs) of the aqueous and ethanolic extracts of (whole) Withania somnifera (L.) Dun. and were determined towards P. aeruginosa (PD1,PD2), Results obtained showed that MIC of ethanolic extract of W. somnifera was (5) mg / ml and of aqueous extract was (175) mg / ml. The effect of Sub – MICs of the aqueous and ethanolic extracts of on the production of hemolysin, elastase and urease was investigated. Sub-MICs of the ethanolic and aqueous extract of W. somnifera had an inhibitory effect and of aqueous extract was (175) mg / ml. The effect of Sub – MICs of the aqueous and ethanolic extracts of on the production of hemolysin, elastase and urease was investigated. Sub-MICs of the ethanolic and aqueous extract of W. somnifera had an inhibitory effect on hemolysin production and a suppressing effect on elastase but no effect on urease production at 100 , 125 , 150 mg / ml for the aqueous extract and 2 , 3 , 4 mg / ml for the ethanolic extract.