DETECTION THE OCCURRENCE OF HELICOBACTER PYLORI AMONG DYSPEPTIC PATIENT AND THEIR SUSCEPTIBILITY TO ANTIBIOTIC AGENTS

Abstract

Fifty dyspeptic patient include 31 female and 19 male were subjected to esophageal gastroduodenoscopy and gastric biopsy and gastric biopsy specimens were taken from antrum and body were applied for microbiological analysis which include : urease test bacterial culture using selective and non selective media and histological examination for detecting H. pylori, and studying their susceptibility to various to antimicrobial agents. Results showed that occurrence of H.pyloria was (100%) among gastric ulcer patient,71% among duodenal ulcer and these with normal endoscopic appearance, as well as, (70%) of gastric patient were found to be infected with H.pylori. Biopsy urease test were proved to be most sensitive method for detecting H.pylori infection when 76% gave positive result for presence of H.pyloria using it, followed by histological examination when 70% of gastric tissues showed the existence of H.pylori organisms. Results also showed that using selective media was batter than not selective media for primary isolation of bacteria, with a recovery rate 61% using brain heart infusion agar and 39% was recovery rate for non selective blood agar.When isolates of H.pylori were subjected to the sensitivity test against (8) antibiotic groups, result, showed that ciprofloxacin was the most effective antibiotic against the isolates when the sensitivity percentage 90%pencilin G,tetracycline, amoxicillin and metronidazal on other hand, were the last Effective antibiotic antibiotics when bacterial sensitivity range between 30% and 90%.