An Improved Janus Green Technique for Detection of Mitochondrial Structure with Electron Microscope

Abstract

Previous studies have shown that Janus Green have a role in mitochondrial localization in light microscopy. In this study, Janus green have been used in electron microscopic technique to demonstrate fine mitochondrial structure. Tissue specimens from mice liver was preceded for electron microscope examination according to Hayat method, 1986. Certain modification was performed to use Janus green with uranyl acetate. The result has shown (hat Janus green gives a good contrasts to the membranous system not only to the mitochondria but, also to other cytoplasmic organelles (rough endoplasmic reticulum and nuclear envelop). Using Janus Green in electron microscopic preparation reflected the interaction between Janus Green and uranyl acetate and gives a good contrast to many cell components