Kinetic Spectrophotometric Methods for the Determination of Chloramphenicol in Pharmaceutical Preparations
Keywords:
chloramphenicol, kinetic spectrophotometry, oxidative–coupling reactionAbstract
Simple and sensitive kinetic methods are described for the determination of chloramphenicol in pure form and pharmaceutical preparations. The methods are based on oxidative – coupling reaction between reduced chloramphenicol (by zinc powder and concentrated hydrochloric acid) with promethazine hydrochloride in the presence of sodium periodate yielding a highly colored product at room temperature, the reaction is followed spectrophotometriclly at λmax= 590 nm. Initial rate and fixed time (at 20 minutes) methods are utilized for concentration determination. The calibration graphs were linear in the concentration ranges (2-20 µg.ml-1) and (0.5-30 µg.ml-1) respectively. The results were validated statistically and checked through recovery studies, and have been applied successfully for the determination of chloramphenicol in commercial dosage formsDownloads
Published
2018-07-05
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Articles
How to Cite
(1)
Kinetic Spectrophotometric Methods for the Determination of Chloramphenicol in Pharmaceutical Preparations. ANJS 2018, 15 (4), 22-30.