Proteus vulgaris من Asparaginaseتنقية وتوصيف انزيم
Keywords:
Asparaginase, Purification, characterization, ProductionAbstract
The enzyme was extracted from Proteus vulgaris and partially purified by many steps including precipitation with ammonium sulfate by using different saturation percentages; the optimum percent was 80% gave value of specific activity 0.86 U/mg protien. then ion exchange chromatography by DEAE-cellulose column, the specific activity 2.7 U/mg protien, and gel filtration using Sephadex G-200 column specific activity 6U/mg protein. Characterization showed that the optimum pH and temperature for enzyme activity 7.5,37Downloads
Published
2012-12-01
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Section
Articles
How to Cite
(1)
Proteus Vulgaris من Asparaginaseتنقية وتوصيف انزيم. ANJS 2012, 15 (4), 43-52.