Proteus vulgaris من Asparaginaseتنقية وتوصيف انزيم

Authors

  • لميس محمد رياض عباس قسم التقنيات الإحيائية ، كمية العموم ، جامعة بغداد.

Keywords:

Asparaginase, Purification, characterization, Production

Abstract

The enzyme was extracted from Proteus vulgaris and partially purified by many steps including precipitation with ammonium sulfate by using different saturation percentages; the optimum percent was 80% gave value of specific activity 0.86 U/mg protien. then ion exchange chromatography by DEAE-cellulose column, the specific activity 2.7 U/mg protien, and gel filtration using Sephadex G-200 column specific activity 6U/mg protein. Characterization showed that the optimum pH and temperature for enzyme activity 7.5,37

Published

2012-12-01

Issue

Section

Articles

How to Cite

(1)
Proteus Vulgaris من Asparaginaseتنقية وتوصيف انزيم. ANJS 2012, 15 (4), 43-52.