Isolation and Identification of Three Bioactive Compounds from Endophytic Fungus Trichoderma sp.

Abstract

The endophytic fungus Trichoderma sp. was isolated from Ocimum basilicum L. plant and cultivated in the laboratory on potato dextrose agar PDA medium. Three bioactive chemical compounds were isolated and purified from culture of Trichoderma sp. using potato dextrose agar and potato dextrose broth. An investigation of the secondary metabolites by using Thin layer chromatography, column chromatography and Gas chromatography technique was carried out ,The molecular weight of purified compounds 1, 2 and 3 were 202 KD, 137 KD and 197 KD respectively and chemical name of compound 1 is1,10-Decanedioic acid, chemical name of compound 2 is Phenol, 4-(ethylamino)-4-Ethylaminophenol and chemical name of compound3 is Pyrimido [5,4-E]-1,2,4-triazine-5,7(4ah,6H)-dione,8,8a-dihydo-4a-hydroxy-6-methyl-. The antibacterial activity of the purified compounds against two bacterial species E.coli and S. aureus were tested by using a disc diffusion agar method reaching to 32,20mm for compound 1and 24,19 mm for compound 2 and 27, 26mm for compound 3. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were determined by the standard serial dilution assay.