عزل وتشخيص بكتريا Helicobacter pylori محلياً من المصابين بالقرح المعوية والمعدية باستخدام وسط انتقائي محوّر ودراسة حساسيتها للمضادات الحيوية

Abstract

Biopsies from 103 Iraqi patients with gastric and duodenal ulcers were cultured by using two selective media for primary isolation of Helicobacter pylori , the first medium was Skirrow selective medium , the second was modified Skirrow selective medium (Al_baldawi) containing brain heart infusion agar supplemented with 5% lysed horse blood&antibiotics: Vancomycin (10µg/ml), Trimethoprim 5µg/ml, polymyxin-B (2.5) IU/ml and Amphotericin-B 5µg /ml.Isolation percentage were 86.3% and 74% by using modified Skirrow selective medium (Al_baldawi) and Skirrow medium respectively .Results showed that number of H. pylori colonies were greater in modified medium than that in Skirrow selective medium , also the results shows less contamination percentage by using modified Skirrow selective medium (Al_baldawi) than Skirro medium. These results indicated superiority of modified medium for primary isolation. Antibiotic sensitivity test for (9) isolates indicates that H. pylori isolates were resistant (100) % to amoxicillin, metronidazol and ciprofloxacin while these isolates were sensitive (100) % to clarithromycin, tetracycline, doxycyclin and cephotaxime. These results clarify the importance of using specific regime therapy which is not absolutely involved a Amoxicillin and metronidazol, the two antibiotic that are used allover the world in gastroduodenal ulcers therapy.