أستخلاص وتنقية حامض اللايبوتكوك جزئيا من بكتريا Enterococcus faecalis المعزولة محليا

Authors

  • خمائل لطفي شاكر قسم علوم الحياة، كلية العلوم ، جامعة بغداد
  • مي طالب فليح قسم علوم الحياة، كلية العلوم ، جامعة بغداد
  • لينة عبد الكريم قسم علوم الحياة، كلية العلوم ، جامعة بغداد

Keywords:

dLTA, استخلاص, حامض اللايبوتكوك, الفينول

Abstract

Fourty seven Enterococcus faecalis isolates were obtained from 300 samples (100 Stool sample, 100 Urine sample, 100 Skin infection sample) at percentages of 28, 15 and 4% respectively. Lipotechoic acid (LTA) was extracted from the isolate Enterococcus faecalis U12.. Extraction was carried out using hot phenol and LTA was partially purified by gel filtration chromatography using Sepharose CL- 4B (1.4×65 cm). The peak of LTA was occupied the fractions from 16-27, the concentration of phosphate was 45.4 μmol ⁄g and the protein percentage was 1.2% of the lyophilized LTA. The sensitizing activity of extracted LTA was 62.5 μg ⁄ml and 15.6 μg ⁄ml for partially purified LTA, with no sensitizing activity for dLTA.

Published

2018-09-02

Issue

Section

Articles

How to Cite

(1)
أستخلاص وتنقية حامض اللايبوتكوك جزئيا من بكتريا Enterococcus Faecalis المعزولة محليا. ANJS 2018, 15 (2), 10-16.