Relation of MDA as Oxidative Stress Marker with Lipid Profile in a Diabetic Patient

Authors

  • Reem M. Azeez Department of Chemistry, College of Science, University of Al-Nahrain, Al-Jadriya, Baghdad, Iraq
  • Wisam K. Al-Hashemi Department of Chemistry, College of Science, University of Al-Nahrain, Al-Jadriya, Baghdad, Iraq. National Diabetes Center, Mustansiriyah University, Baghdad, Iraq.
  • Tawfeeq F. R. Al-Auqbi National Diabetes Center, Mustansiriyah University, Baghdad, Iraq.
  • Jamel Jebali Higher Institute of Biotechnology of Monastir, University of Monastir, Monastir, Tunisia.

Keywords:

T2DM, Malondialdehyde, Oxidative Stress, Obesity

Abstract

Type-2 diabetes mellitus (T2DM) is a disease accompanied by induced oxidative stress (OS) conditions. A reduction in antioxidant activity characterizes the OS conditions. Antioxidants protect cell components from the harmful effects of reactive oxygen species (ROS). The oxidation of the lipid components by ROS has the potential to generate malondialdehyde (MDA). The assessment of MDA (which is a byproduct of lipid peroxidation) may serve as a sign of oxidative damage. This can shed light on the impact of ROS on lipids. This study aims to elucidate the correlation between MDA oxidative stress biomarkers and lipids in individuals diagnosed with type-2 diabetes mellitus. The study comprises two case groups of 30 diabetic patients, 30 obese patients, and 30 healthy patients as the control group. The levels of MDA in the plasma specimens are measured. The serum glucose, serum lipid, and glycated haemoglobin (HbA1c) are evaluated for these groups. The results among patients with and without T2DM are analyzed with regard to age and BMI. The concentration of the MDA is [17.08 ± 1.989 (nmol/ml)] for diabetic patients, [16.08 ± 2.049 (nmol/ml)] for obese patients, and [12.12 ± 1.508 (nmol/ml)] for control patients. A statistically significant difference of p <0.0001 is observed in the elevated levels of MDA among T2DM patients. As compared to healthy control participants, there is no significant difference between T2DM and obese patients. It is concluded that the MDA can be utilized for observing the oxidative stress related to T2DM. The data indicate that utilising MDA could help predict the oxidative environment.

References

Marseglia. L; “Oxidative Stress in Obesity: a Critical Component in Human Diseases”. Int. J. Mol. Sci.,16 (1): 378–400, 2014.

Manna, P.; Jain S.K.; “Obesity, Oxidative Stress, Adipose Tissue Dysfunction, and the Associated Health Risks: Causes and Therapeutic Strategies”. Metab. Syndr. Relat. Disord., 13 (10): 423–444, 2015.

Singh U.N.; Kumar S.; Dhakal S.; “Study of Oxidative Stress in Hypercholesterolemia”. Int. J. Contemp. Med. Res., 4(5): 1204–1207, 2017.

Oliveira J.S.; Silva A.A.N.; Silva V.A.; “Phytotherapy in Reducing Glycemic Index and Testicular Oxidative Stress Resulting from Induced Diabetes: a Review”. Brazilian J. Biol., 77: 68–78, 2016.

Merhan O.; Bozukluhan K.; Mushap K.; Büyük F.; Özden Ö.; Kükürt A.; et al., “Investigation of Oxidative Stress Index and Lipid Profile in Cattle with Brucellosis”. Kafkas Üniversitesi Vet. Fakültesi Derg., 23(6), 2017.

Hussain W.F.; Al-Hashemi W.K.H.H.; “Effect of Oxidative Stress on Iraqi Rheumatoid Arthritis Patients”. Al-Nahrain J. Sci., 21 (2): 28–31, 2018.

Kotb, A.; Al-Azzam K.M.; “Effect of Vitamin C on Blood Glucose and Glycosylated Hemoglobin in Type II Diabetes Mellitus”. World J Anal Chem, 3 (1): 6–8, 2015.

Naqvi S.; “Correlation Between Glycated Hemoglobin and Triglyceride Level in Type 2 Diabetes Mellitus”. Cureus, 9 (6), 2017.

Haghighatpanah, M.; Thunga, G.; Khare, S.; Mallayasamy, S.; “Correlation of Glycosylated Hemoglobin Levels with Fasting and Postprandial Glucose in South Indian Type 2 Diabetic Patients”. Int. J. Pharm. Pharm. Sci., 8 (8): 285–288, 2016.

Nielsen, F.; Mikkelsen, B.B.; Nielsen, J.B.; Andersen, H.R.; Grandjean, P.; et al., “Plasma Malondialdehyde as Biomarker for Oxidative Stress: Reference Interval and Effects of Life-Style Factors”. Clin. Chem., 43 (7): 1209–1214, 1997.

Jawad, A.H.; Al-Hashemi, W.K.H.; “Study of some Markers of Oxidative Stress Statues in Cardiovascular Disease Patients”. Al-Nahrain J. Sci., 18 (3): 38–43, 2015.

Sunita, R.; Sahidan, S.; Hidayat, R.; “Evaluation of Malondialdehyde in Type 2 Diabetes Mellitus Patients as Oxidative Stress Markers in Bengkulu Population”. Biosci. Med. J. Biomed. Transl. Res., 4 (3): 45–54, 2020.

Erdogan, H.M.; Karapehlivan, M.; Citil, M.; Atakisi, O.; Uzlu, E.; Unver, A.; et al., “Serum Sialic Acid and Oxidative Stress Parameters Changes in Cattle with Leptospirosis”. Vet. Res. Commun., 32: 333–339, 2008.

Forbes, J.M.; Cooper, M.E.; “Mechanisms of Diabetic Complications”. Physiol. Rev., 93 (1): 137–188, 2013.

Frohnert, B.I.; Bernlohr, D.A.; “Protein Carbonylation, Mitochondrial Dysfunction, and Insulin Resistance”. Adv. Nutr., 4 (2): 157–163, 2013.

Guo, L.; Chen, Z.; Amarnath, V.; Davies S.S.; “Identification of Novel Bioactive Aldehyde-Modified Phosphatidylethanolamines Formed by Lipid Peroxidation”. Free Radic. Biol. Med., 53(6): 1226–1238, 2012.

Albargawi, M.; Snethen, J.; Gannass A.A.L.; Kelber, S.; “Perception of Persons with Type 2 Diabetes Mellitus in Saudi Arabia”. Int. J. Nurs. Sci., 3 (1): 39–44, 2016.

Jayasekharan, V.P.; Ramya, R.; Rajkumar, K.; Kumar, T.D.; Nandhini, G.; Kumar, S.S.; et al., “Estimation of Nitric Oxide and Malondialdehyde in Serum and Saliva of Patients with Oral Lichen Planus”. SRM J. Res. Dent. Sci., 5 (4): 230, 2014.

Khan, M.W.A.; Banga, K.; Khan, W.A.; “Gluco-Oxidation of Proteins in the Etiology of Diabetic Retinopathy”. Diabet. Retin. InTech,: 31–52, 2012.

Kumawat, M.; “Antioxidant Enzymes and Lipid Peroxidation in Type 2 Diabetes Mellitus Patients with and without Nephropathy”. N. Am. J. Med. Sci., 5 (3): 213, 2013.

Kundu, D.; Roy, A.; Mandal T.; Bandyopadhyay U.; Ghosh E. and Ray D.; et al., “Relation of Iron Stores to Oxidative Stress in Type 2 Diabetes”. Niger. J. Clin. Pract., 16 (1): 100–103, 2013.

Kurtul, N.; Gökpınar, E.; “Salivary Lipid Peroxidation and Total Sialic Acid Levels in Smokers and Smokeless Tobacco Users as Maraş Powder”. Mediators Inflamm., 2012, 2012.

Kutmon, M.; Evelo, C.T.; Coort S.L.; “A network Biology Workflow to Study Transcriptomics Data of the Diabetic Liver”. BMC Genomics, 15: 1–10, 2014.

Ladgotra, A.; Verma P.; Raj S.S.; “Estimation of Salivary and Serum Biomarkers in Diabetic and non Diabetic Patients-a Comparative Study”. J. Clin. diagnostic Res. JCDR, 10 (6): ZC56, 2016.

Isola, G.; Polizzi, A.; Santonocito, S.; Alibrandi, A.; Ferlito, S.; et al., “Expression of Salivary and Serum Malondialdehyde and Lipid Profile of Patients with Periodontitis and Coronary Heart Disease”. Int. J. Mol. Sci., 20 (23): 6061, 2019.

Ito, F.; Sono,Y.; Ito, T.; “Measurement and Clinical Significance of Lipid Peroxidation as a Biomarker of Oxidative Stress: Oxidative Stress in Diabetes, Aherosclerosis, and Chronic Inflammation”. Antioxidants, 8 (3): 72, 2019.

Morales, M.; Munné-Bosch S.; “Malondialdehyde: Facts and Artifacts”. Plant Physiol., 180 (3): 1246–1250, 2019.

Li, Z.; Geng, Y.-N.; Jiang, J.-D.; Kong, W.-J.; “Antioxidant and Anti-Inflammatory Activities of Berberine in the Treatment of Diabetes Mellitus”. Evidence-based Complement. Altern. Med., 2014, 2014.

Klisic, A.; “Relationship Between Oxidative Stress, Inflammation and Dyslipidemia with Fatty Liver Index in Patients with Type 2 Diabetes Mellitus”. Exp. Clin. Endocrinol. Diabetes, 126 (06): 371–378, 2018.

Tangvarasittichai, S.; “Oxidative Stress, Insulin Resistance, Dyslipidemia and Type 2 Diabetes Mellitus”. World J. Diabetes, 6 (3): 456, 2015.

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Published

2023-09-18

Issue

Vol. 26 No. 3 (2023): September

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Articles

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Copyright (c) 2024 Reem M. Azeez , Wisam K. Al-Hashemi , Tawfeeq F. R. Al-Auqbi , Jamel Jebali

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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(1)
Relation of MDA As Oxidative Stress Marker With Lipid Profile in a Diabetic Patient. ANJS 2023, 26 (3), 12-17.