Challenges Facing Us in DNA Analysis from Human Hair Samples: A Review
DOI:
https://doi.org/10.22401/w8999a02Keywords:
Crime scene , DNA analysis , Forensic analysis , Human hair in criminology , Mitochondrial DNAAbstract
Human hair is a valuable tool in criminology, aiding in population studies through statistics and DNA analysis. It's easily identifiable at crime scenes, often clinging to clothing, carpets, and various surfaces, including animal fur, through a process known as secondary transfer. Forensic analysis of hair evidence serves several purposes; it can help establish the possibility of a connection between a suspect and a crime scene or between a suspect and a victim. It can demonstrate that there is no evidence linking the perpetrator to the crime scene or the suspect to the victim. While microscopic hair analysis cannot definitively identify a specific individual as the source of the hair, it does provide a solid basis for association. The wealth of macroscopic and microscopic details available in hair examination can also provide strong evidence for the defense. To understand the challenges of conducting DNA tests on hair, it's essential to study hair structure and composition. DNA in hair is not evenly distributed throughout every part of the hair; it exists in both nuclear and mitochondrial forms.
References
Suenaga, E.; Nakamura, H.; “Evaluation of three methods for effective extraction of DNA from human hair”. J. Chromatogr. A., 820(1): 137-141; 2005.
Ghatak, S.; Muthukumaran, R.B.; Nachimuthu, S.K.; “A simple method of genomic DNA extraction from human samples for PCR-RFLP analysis”. J. Biomol. Tech., 24(4): 224, 2013.
Bisbing, R.E.; Wolner, M.F.; ”Microscopical discrimination of twins' head hair”. J. Forensic Sci., 29(3): 780-786, 1984.
Cortes-Figueiredo, F.; Carvalho, F.S.; Fonseca, A.C.; Paul, F.; Ferro, J.M.; Schönherr, S.; Morais, V.A.; “From Forensics to Clinical Research: Expanding the Variant Calling Pipeline for the Precision ID mtDNA Whole Genome Panel”. Int. J. Mol. Sci., 22(21):12031, 2021.
Cortellini, V.; Carobbio A.; Brescia G.; Cerri N.; Verzeletti A.; “A Comparative Study of Human and Animal Hairs: Microscopic Hair Comparison and Cytochrome: c: Oxidase I Species Identification”. J. Forensic Sci. Med., 5(1): 20-3, 2019.
Lanning, K.; Michaud, A.; Bisbing, R.; Springer, F.; Tridico, D.S.; “Scientific Working Group on Materials Analysis Position oFn Hair Evidence”. J. Forensic Sci., 54:1198–1202, 2018.
Houck, M.M.; Budowle, B.; “Correlation of microscopic and mitochondrial DNA hair comparisons”. J. Forensic Sci., 47(5): 1-4, 2002.
Suenaga, E.; Nakamura, H.; “Evaluation of three methods for effective extraction of DNA from human hair”. J. Chromatogr. A; 820(1); 137-141; 2005.
Linch, C.A.; “The ultrastructure of tissue attached to telogen hair roots”. J. Forensic Sci.; 53(6), 1363-1366; 2008.
Wang, X.; Shi, Y.; Zhou, Q.; Liu, X.; Xu, S.; Lei, T.; “Detailed histological structure of human hair follicle bulge region at different ages: a visible niche for nesting adult stem cells”. J. Huazhong Univ. Sci. Technolog Med. Sci., 32(5): 648-656, 2012.
Buffoli, B.; Rinaldi, F.; Labanca, M.; Sorbellini, E; Trink, A.; Guanziroli, E.; Rodella, L.F.; ”The human hair: from anatomy to physiology”. Int. J. Dermatol.,53(3): 331-341, 2014.
Wolfram, L.J.; ”Human hair: a unique physicochemical composite”. J. Am. Acad. Dermatol, 48(6): S106-S114, 2003.
Alvarez-Cubero, M.J.; Saiz, M.; Martinez-Gonzalez, L.J.; Alvarez, J.C.; Eisenberg, A.J.; Budowle, B.; Lorente, J.A.; “Genetic identification of missing persons: DNA analysis of human remains and compromised samples”. Pathobiology, 79,(5): 2012.
Lanning, K.; Michaud, A.; Bisbing, R.; Springer, F.; Tridico, D.S.; “Scientific Working Group on Materials Analysis Position on Hair Evidence”. J. Forensic Sci., 54(5): 1198–1202, 2009.
Melton, T.; Holland, C.; Holland, M.; “Forensic mitochondria DNA analysis: Current practice and future potential”. Forensic Sci. Rev., 24(2): 101, 2012.
Machado, H.; Granja, R.; “DNA Technologies in Criminal Investigation and Courts. In: Forensic Genetics in the Governance of Crime. Palgrave Pivot,Singapore,(2020).
Finaughty, C.; Heathfield, L.J.; Kemp, V.; Márquez-Grant, N.; “Forensic DNA extraction methods for human hard tissue: A systematic literature review and meta-analysis of technologies and sample type”. Forensic Sci. Int. Genet., 63: 102818, 2023.
Just, R.S.; Irwin, J.A.; Parson, W.; “Mitochondrial DNA heteroplasmy in the emerging field of massively parallel sequencing”. Forensic Sci. Int. Genet. 18: 131-139, 2015.
Higuchi, R.; von-Beroldingen, C.H.; Sensabaugh, G.F.; Erlich, H.A.; “DNA typing from single hairs”. Nature, 332(6164): 543-546, 1988.
Campos, P.F.; Gilbert, M.T.P.; “DNA Extraction from Keratin and Chitin”. Methods Mol. Biol.,1963:57-63, 2019.
Wilson, M.R.; Polanskey, D.; Butler, J.; Di-Zinno, J.A.; Replogle, J.; Budowle, B.; “Extraction, PCR amplification and sequencing of mitochondrial DNA from human hair shafts”. Biotechniques, 18, 662-669, 1995.
Higuchi, R.; von-Beroldingen, C.H.; Sensabaugh, G.F.; Erlich, H.A.; “DNA typing from single hairs”. Nature, 332(6164): 543-546, 1988.
Sullivan, K.M.; Hopgood, R.; Gill, P.; ”Identification of human remains by amplification and automated sequencing of mitochondrial DNA”. Int. J. Legal Med.,105(2): 83-86, 1992.
Grisedale, K.S.; Murphy, G.M.; Brown, H.; Wilson, M.R.; Sinha, S.K.; “Successful nuclear DNA profiling of rootless hair shafts: a novel approach”. Int. J. Legal Med., 132(1): 107–115, 2018.
Chung, D.T.; Drábek, J.; Opel, K.L.; Butler, J.M.; McCord, B.R.; “A study on the effects of degradation and template concentration on the amplification efficiency of the STR Miniplex primer sets”. J. Forensic Sci., 49(4): 1-8, 2004.
Coble, M.D.; Butler, J.M.; “Characterization of new miniSTR loci to aid analysis of degraded DNA”. J. Forensic Sci., 50(1): 43–53, 2005.
Alberts, C.C.; Ribeiro-Paes, J.T.; Aranda-Selverio, G.; Cursino-Santos, J.R.; Moreno-Cotulio, V.R.; Oliveira, A.L.D.; Souza, E.B.D.; “DNA extraction from hair shafts of wild Brazilian felids and canids”. Genet. Mol. Res., 9: 2429-2435, 2010.
Schneider, P.M.; Bender, K.; Mayr, W.R.; Parson, W.; Hoste, B.; Decorte, R.; Greenhalgh, M.; “STR analysis of artificially degraded DNA— results of a collaborative European exercise”. Forensic Sci. Int., 139(2-3): 123-134, 2004.
Suenaga, E.; Nakamura, H.; “Evaluation of three methods for effective extraction of DNA from human hair”. J. Chromatogr. A., 820(1): 137-141, 2005.
Romeika, J.M.; Yan, F.; “ Recent advances in forensic DNA analysis”. J. Forensic Sci., S13: 001, 2013.
Suenaga, E.; Nakamura, H.; “Evaluation of three methods for effective extraction of DNA from human hair”. J. Chromatogr. B., 820(1):137-141, 2005.
Allen, M.; Engström, A.S.; Meyers, S.; Handt, O.; Saldeen, T.; von-Haeseler, A.; Gyllensten, U.; “Mitochondrial DNA sequencing of shed hairs and saliva on robbery caps: sensitivity and matching probabilities”. J. Forensic Sci., 43(3): 453-464, 1998.
Sujirachato, K.; Tirawatnapong, S.; Mimae, N.; Inon, S.; Kaewdouengdee, A.; Worasuwannarak, W.; “The Study of the Yield of DNA Extracted From Hairs of Postmortem Cases”. Ramathibodi Med. J., 43: 9–18, 2020
Anderson, S.; Bankier, A.T.; Barrell, B.G.; de-Bruijn, M.H.; Coulson, A.R.; Drouin, J.; Young, I.G.; “ Sequence and organization of the humanmitochondrial.genome”. Nature, 290(5806): 457-465, 1981.
Nilsson, M.; “ Sequencing of mtDNA in Shed Hairs: A Retrospective Analysis of Material from Forensic Cases and a Pre-Screening Method". Open Forensic Sci. J., 5(1): 13–22, 2012.
Lindahl, T.; Andersson, A.; “Rate of chain breakage at apurinic sites in double-stranded deoxyribonucleicacid”. J. Biochem., 11(19): 3618 - 3623; 1972.
Just, R.S.; Irwin, J.A.; Parson, W.; “Mitochondrial DNA heteroplasmy in the emerging field of massively parallel sequencing”. Forensic Sci. Int. Genet., 18: 131-139, 2015.
Higuchi, R.; von-Beroldingen, C.H.; Sensa-baugh, G.F.; Erlich, H.A.; “DNA typing from single hairs”. Nature, 332(6164): 543-546, 1988.
Anderson, T.D.; Ross, J.P.; Roby, R.K.; Lee, D.A.; Holland, M.M.; “A validation study for the extraction and analysis of DNA from human nail material and its application to forensic casework”. J. Forensic Sci., 44(5): 1053-1056, 1999.
Templeton, J.E.; Brotherton, P.M.; Llamas, B.; Soubrier, J.; Haak, W.; Cooper, A.; Austin, J.J.; “DNA capture and next-generation sequencing can recover whole mitochondrial genomes from highly degraded samples for human identification”. Investig. Genet. 4(1): 1-13, 2013.
Kuznetsov, A.V.; Ausserlechner, M.J.; “Research of Mitochondrial Function, Structure, Dynamics and Intracellular Organization”. Int. J. Mol. Sci., 24(1): 886, 2023.
Ambers, A.; Wiley, R.; Novroski, N.; Budowle, B.; “Direct PCR amplification of DNA from human bloodstains, saliva, and touch samples collected with microFLOQ® swabs”. Forensic Sci. Int. Genet., 32: 80–87, 2018.
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Copyright (c) 2024 Halah K. Ibrahim Al-Sammaraie, Reem Husam Al-Tabra, Asia Abdul Lateef Mahdi, Mayyahi Mohammed T. Jaber, May R. Jaafar, Mohammed Mahdi Al–Zubaidi, Asmaa A. Jawad
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